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A. MS2 phage clearance at varying rotor speeds; B. Images of filter surface after centrifugal ultrafiltration at 5000 rpm, including nanocellulose‐based filter paper (upper panel) and support paper (lower panel); C. <t>ΦX174</t> phage clearance at varying rotor speeds; D. LRV MS2 phage clearance to half‐volume at varying rotor speeds. Dotted line represents 4 log 10 virus clearance threshold. Dotted line represents 4 log 10 virus clearance threshold.
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A. MS2 phage clearance at varying rotor speeds; B. Images of filter surface after centrifugal ultrafiltration at 5000 rpm, including nanocellulose‐based filter paper (upper panel) and support paper (lower panel); C. <t>ΦX174</t> phage clearance at varying rotor speeds; D. LRV MS2 phage clearance to half‐volume at varying rotor speeds. Dotted line represents 4 log 10 virus clearance threshold. Dotted line represents 4 log 10 virus clearance threshold.
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A. MS2 phage clearance at varying rotor speeds; B. Images of filter surface after centrifugal ultrafiltration at 5000 rpm, including nanocellulose‐based filter paper (upper panel) and support paper (lower panel); C. <t>ΦX174</t> phage clearance at varying rotor speeds; D. LRV MS2 phage clearance to half‐volume at varying rotor speeds. Dotted line represents 4 log 10 virus clearance threshold. Dotted line represents 4 log 10 virus clearance threshold.
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A. MS2 phage clearance at varying rotor speeds; B. Images of filter surface after centrifugal ultrafiltration at 5000 rpm, including nanocellulose‐based filter paper (upper panel) and support paper (lower panel); C. <t>ΦX174</t> phage clearance at varying rotor speeds; D. LRV MS2 phage clearance to half‐volume at varying rotor speeds. Dotted line represents 4 log 10 virus clearance threshold. Dotted line represents 4 log 10 virus clearance threshold.
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viruses bacteriophage phix174  (ATCC)
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A. MS2 phage clearance at varying rotor speeds; B. Images of filter surface after centrifugal ultrafiltration at 5000 rpm, including nanocellulose‐based filter paper (upper panel) and support paper (lower panel); C. <t>ΦX174</t> phage clearance at varying rotor speeds; D. LRV MS2 phage clearance to half‐volume at varying rotor speeds. Dotted line represents 4 log 10 virus clearance threshold. Dotted line represents 4 log 10 virus clearance threshold.
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φx174  (ATCC)
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A. MS2 phage clearance at varying rotor speeds; B. Images of filter surface after centrifugal ultrafiltration at 5000 rpm, including nanocellulose‐based filter paper (upper panel) and support paper (lower panel); C. <t>ΦX174</t> phage clearance at varying rotor speeds; D. LRV MS2 phage clearance to half‐volume at varying rotor speeds. Dotted line represents 4 log 10 virus clearance threshold. Dotted line represents 4 log 10 virus clearance threshold.
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A. MS2 phage clearance at varying rotor speeds; B. Images of filter surface after centrifugal ultrafiltration at 5000 rpm, including nanocellulose‐based filter paper (upper panel) and support paper (lower panel); C. ΦX174 phage clearance at varying rotor speeds; D. LRV MS2 phage clearance to half‐volume at varying rotor speeds. Dotted line represents 4 log 10 virus clearance threshold. Dotted line represents 4 log 10 virus clearance threshold.

Journal: Biotechnology Journal

Article Title: Analytical Centrifugal Ultrafiltration as a Tool for High Throughput Process Development in Virus Removal Filtration

doi: 10.1002/biot.70227

Figure Lengend Snippet: A. MS2 phage clearance at varying rotor speeds; B. Images of filter surface after centrifugal ultrafiltration at 5000 rpm, including nanocellulose‐based filter paper (upper panel) and support paper (lower panel); C. ΦX174 phage clearance at varying rotor speeds; D. LRV MS2 phage clearance to half‐volume at varying rotor speeds. Dotted line represents 4 log 10 virus clearance threshold. Dotted line represents 4 log 10 virus clearance threshold.

Article Snippet: Bacteriophages ΦX174 (ATCC 13706‐B1) and MS2 (ATCC 15597‐B1), along with their respective host Escherichia coli (Migula) Castellani and Chalmers bacterial strains C (ATCC 13706) and C‐3000 (ATCC 15597), were procured from ATCC (Manassas, VA).

Techniques: Virus